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STEMCELL Technologies Inc mouse recombinant leukemia inhibitory factor (lif)
Mouse Recombinant Leukemia Inhibitory Factor (Lif), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+recombinant+leukemia+inhibitory+factor+(lif)/murine+recombinant+leukemia+inhibitory+factor/pmc11161795__pnas__2318740121__sapp-72-44-51
Average 90 stars, based on 1 article reviews
mouse recombinant leukemia inhibitory factor (lif) - by Bioz Stars, 2026-10
90/100 stars

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Cell Culture:

Article Title: Bioengineering embryonic stem cell microenvironments for exploring inhibitory effects on metastatic breast cancer cells.
Article Snippet: The recreation of an in vitro microenvironment to understand and manipulate the proliferation and migration of invasive breast cancer cells may allow one to put a halt to their metastasis capacity.. Invasive cancer cells have been linked to embryonic stem (ES) cells as they possess certain similar characteristics and gene signatures.. Embryonic microenvironments have the potential to reprogram cancer cells into a less invasive phenotype and help elucidate tumorigenesis and metastasis.

Article Title: Semi-continuous Flow Electroporation Chip for High Throughput Transfection on Mammalian Cells
Article Snippet: Mouse embryonic stem (mES) cells were donated by StemCell Technologies (Vancouver, Canada). .. The mES cells were cultured on gelatin-coated tissue culture flasks and maintained in an undifferentiated state using Dulbecco’s Modified Eagle’s Medium (DMEM with 4.5 g/L D-glucose) supplemented with 15%(v/v) fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin, 0.1 mM non-essential amino acids, 10 ng/mL murine recombinant leukemia inhibitory factor (LIF; these reagents were from StemCell Technologies, Vancouver, Canada), 0.1 mM monothioglycerol, 2 mM L-glutamine (Sigma Aldrich, St. Louis, MO) and 1 mM sodium pyruvate (Invitrogen) at 37°C in a humidified atmosphere containing 5% CO 2 . .. The plasmid pGFP (gWizTM GFP, 5757 bp) was obtained from Aldevron.

Article Title: One dimensional self-assembly of mouse embryonic stem cells using an array of hydrogel microstrands
Article Snippet: Mouse ES cell culture Mouse CCE ES cells were obtained from StemCell Technologies (Vancouver, Canada) [ 57 , 58 ]. .. Mouse ES cells were cultured in gelatin coated flasks in a standard cell culture incubator at 37 °C, 5% CO 2 and maintained in an undifferentiated state in growth medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM with 4.5 g/l D-glucose) supplemented with 15% (v/v) fetal bovine serum (FBS), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 mM non-essential amino acids, 10 ng/ml murine recombinant leukemia inhibitory factor (LIF; StemCell Technologies, Vancouver, Canada), 0.1 mM monothioglycerol, and 2 mM L-glutamine and 1 mM sodium pyruvate (Sigma Aldrich, St. Louis, MO). ..

Modification:

Article Title: Bioengineering embryonic stem cell microenvironments for exploring inhibitory effects on metastatic breast cancer cells.
Article Snippet: The recreation of an in vitro microenvironment to understand and manipulate the proliferation and migration of invasive breast cancer cells may allow one to put a halt to their metastasis capacity.. Invasive cancer cells have been linked to embryonic stem (ES) cells as they possess certain similar characteristics and gene signatures.. Embryonic microenvironments have the potential to reprogram cancer cells into a less invasive phenotype and help elucidate tumorigenesis and metastasis.

Article Title: Semi-continuous Flow Electroporation Chip for High Throughput Transfection on Mammalian Cells
Article Snippet: Mouse embryonic stem (mES) cells were donated by StemCell Technologies (Vancouver, Canada). .. The mES cells were cultured on gelatin-coated tissue culture flasks and maintained in an undifferentiated state using Dulbecco’s Modified Eagle’s Medium (DMEM with 4.5 g/L D-glucose) supplemented with 15%(v/v) fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin, 0.1 mM non-essential amino acids, 10 ng/mL murine recombinant leukemia inhibitory factor (LIF; these reagents were from StemCell Technologies, Vancouver, Canada), 0.1 mM monothioglycerol, 2 mM L-glutamine (Sigma Aldrich, St. Louis, MO) and 1 mM sodium pyruvate (Invitrogen) at 37°C in a humidified atmosphere containing 5% CO 2 . .. The plasmid pGFP (gWizTM GFP, 5757 bp) was obtained from Aldevron.

Article Title: One dimensional self-assembly of mouse embryonic stem cells using an array of hydrogel microstrands
Article Snippet: Mouse ES cell culture Mouse CCE ES cells were obtained from StemCell Technologies (Vancouver, Canada) [ 57 , 58 ]. .. Mouse ES cells were cultured in gelatin coated flasks in a standard cell culture incubator at 37 °C, 5% CO 2 and maintained in an undifferentiated state in growth medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM with 4.5 g/l D-glucose) supplemented with 15% (v/v) fetal bovine serum (FBS), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 mM non-essential amino acids, 10 ng/ml murine recombinant leukemia inhibitory factor (LIF; StemCell Technologies, Vancouver, Canada), 0.1 mM monothioglycerol, and 2 mM L-glutamine and 1 mM sodium pyruvate (Sigma Aldrich, St. Louis, MO). ..

Article Title: 3D brown adipogenesis to create “Brown-Fat-in-Microstrands”
Article Snippet: .. This maintenance medium consisted of Dulbecco’s Modified Eagle’s Medium (DMEM 4.5 g/l D-glucose), enhanced with 15% (v/v) fetal bovine serum (FBS), 10 ng/ml murine recombinant leukemia inhibitory factor (LIF; StemCell Technologies), 0.1 mM non-essential amino acids, 0.1 mM monothioglycerol, 100 U/ml penicillin, 100 μg/ml streptomycin, and 2 mM L-glutamine, and 1 mM sodium pyruvate (Sigma-Aldrich, St Louis, MO). ..

Recombinant:

Article Title: Bioengineering embryonic stem cell microenvironments for exploring inhibitory effects on metastatic breast cancer cells.
Article Snippet: The recreation of an in vitro microenvironment to understand and manipulate the proliferation and migration of invasive breast cancer cells may allow one to put a halt to their metastasis capacity.. Invasive cancer cells have been linked to embryonic stem (ES) cells as they possess certain similar characteristics and gene signatures.. Embryonic microenvironments have the potential to reprogram cancer cells into a less invasive phenotype and help elucidate tumorigenesis and metastasis.

Article Title: Semi-continuous Flow Electroporation Chip for High Throughput Transfection on Mammalian Cells
Article Snippet: Mouse embryonic stem (mES) cells were donated by StemCell Technologies (Vancouver, Canada). .. The mES cells were cultured on gelatin-coated tissue culture flasks and maintained in an undifferentiated state using Dulbecco’s Modified Eagle’s Medium (DMEM with 4.5 g/L D-glucose) supplemented with 15%(v/v) fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin, 0.1 mM non-essential amino acids, 10 ng/mL murine recombinant leukemia inhibitory factor (LIF; these reagents were from StemCell Technologies, Vancouver, Canada), 0.1 mM monothioglycerol, 2 mM L-glutamine (Sigma Aldrich, St. Louis, MO) and 1 mM sodium pyruvate (Invitrogen) at 37°C in a humidified atmosphere containing 5% CO 2 . .. The plasmid pGFP (gWizTM GFP, 5757 bp) was obtained from Aldevron.

Article Title: One dimensional self-assembly of mouse embryonic stem cells using an array of hydrogel microstrands
Article Snippet: Mouse ES cell culture Mouse CCE ES cells were obtained from StemCell Technologies (Vancouver, Canada) [ 57 , 58 ]. .. Mouse ES cells were cultured in gelatin coated flasks in a standard cell culture incubator at 37 °C, 5% CO 2 and maintained in an undifferentiated state in growth medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM with 4.5 g/l D-glucose) supplemented with 15% (v/v) fetal bovine serum (FBS), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 mM non-essential amino acids, 10 ng/ml murine recombinant leukemia inhibitory factor (LIF; StemCell Technologies, Vancouver, Canada), 0.1 mM monothioglycerol, and 2 mM L-glutamine and 1 mM sodium pyruvate (Sigma Aldrich, St. Louis, MO). ..

Article Title: 3D brown adipogenesis to create “Brown-Fat-in-Microstrands”
Article Snippet: .. This maintenance medium consisted of Dulbecco’s Modified Eagle’s Medium (DMEM 4.5 g/l D-glucose), enhanced with 15% (v/v) fetal bovine serum (FBS), 10 ng/ml murine recombinant leukemia inhibitory factor (LIF; StemCell Technologies), 0.1 mM non-essential amino acids, 0.1 mM monothioglycerol, 100 U/ml penicillin, 100 μg/ml streptomycin, and 2 mM L-glutamine, and 1 mM sodium pyruvate (Sigma-Aldrich, St Louis, MO). ..

Article Title: Transplantation of human glial-restricted neural precursors into injured spinal cord promotes functional and sensory recovery without causing allodynia.
Article Snippet: Background aims.. Traumatic injuries of the central nervous system cause damage and degeneration of specifi c cell populations with subsequent functional loss.. Cell transplantation is a strategy to treat such injuries by replacing lost or damaged cell populations.

other:

Article Title: Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro.
Article Snippet: The interaction between embryonic and extraembryonic tissues is critical in natural mouse embryogenesis.. Here, to enable such interaction in vitro, we describe a protocol to assemble a complete mouse embryo model using mouse embryonic stem cells and induced embryonic stem cells to express Cdx2 (or trophoblast stem cells) and Gata4 to reconstitute the epiblast, extraembryonic ectoderm and visceral endoderm lineages, respectively.. The resulting complete embryo models recapitulate development from embryonic day 5.0 to 8.5, generating advanced embryonic and extraembryonic tissues that develop through gastrulation to initiate organogenesis to form a head and a beating heart structure as well as a yolk sac and chorion.

Article Title: Receptor interacting protein kinase 3 (RIP3) regulates iPSCs generation through modulating cell cycle progression genes
Article Snippet: Recombinant mouse leukemia inhibitory factor (LIF) (Cat. # 02740) was purchased from Stem Cell Technologies (Cambridge, MA).

Article Title:
Article Snippet: 166 Neuronal differentiation of mouse embryonic stem cells (mESCs) 167 mESCs were expanded on 0.1% gelatin coated dishes in DMEM (1X)+GlutaMAX media (Thermo Fisher, 168 #10566-016) supplemented with 15% inactivated fetal bovine serum (FBS) (Thermo Fisher, #16141-079), 169 1mM sodium pyruvate (Thermo Fisher, #11360-070), mouse recombinant leukemia inhibitory factor (LIF) 170 (STEMCELL Technologies, #78056.2), 1X MEM Non-Essential Amino Acids (Thermo Fisher, #11140-050), 171 0.1 mM 2-Mercaptoethanol (Thermo Fisher, #31350-010), 100 U/ml penicillin & streptomycin (Thermo 172 Fisher, #15140-122).



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Image Search Results


Journal: eLife

Article Title: An in vitro approach reveals molecular mechanisms underlying endocrine disruptor-induced epimutagenesis

doi: 10.7554/eLife.93975

Figure Lengend Snippet:

Article Snippet: Chemical compound , Leukemia inhibitory factor (LIF) , Millipore Sigma , ESG1107 , ESGRO Recombinant Mouse LIF Protein.

Techniques: Modification, Recombinant, Derivative Assay, Knock-Out, Saline, Control, DNA Methylation Assay, One Step RT-PCR, SYBR Green Assay, Methylation, Isolation, Software, Microscopy